Publication Type:

Journal Article

Source:

International Journal of Biological Macromolecules, Volume 44, Number 4, p.333-337 (2009)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-62949203849&partnerID=40&md5=e9cd84be2ae6f8f1ebc467128ffc944f

Keywords:

alpha chitin, article, Artificial, biocompatibility, body fluid, Calcium phosphate, cell adhesion, Cell Line, cell membrane, Cells, chitin, chitin derivative, drug activity, Electron, enzyme degradation, gelatin, human, human cell, Humans, hydrogel, Hydrogels, incubation time, Membranes, microscopy, osteoblast, Scanning, scanning electron microscopy, simulation, swelling, tissue engineering, unclassified drug, X ray diffraction, X-Ray Diffraction

Abstract:

The chitin/gelatin composite membranes were prepared by mixing of chitin hydrogel with gelatin. The prepared composite membranes were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical, swelling, enzymatic degradation and thermal studies. The XRD pattern of the chitin/gelatin composite membranes showed almost the same pattern as α-chitin. The bioactivity studies of these chitin/gelatin membranes were carried out with the simulated body fluid solution (SBF) for 7, 14 and 21 days followed by the characterization with the scanning electron microscopy (SEM) and Energy Dispersive Spectrum (EDS) studies. The SEM and EDS studies confirmed the formation of calcium phosphate layer on the surface of chitin/gelatin membranes. Biocompatibility of the chitin/gelatin membrane was assessed using human MG-63 osteoblast-like cells. After 48 h of incubation, it was found that the cells had attached and completely covered the membrane surface. Thus, the prepared chitin/gelatin membranes are bioactive and are suitable for cell adhesion suggesting that these membranes can be used for tissue-engineering applications. © 2009 Elsevier B.V. All rights reserved.

Notes:

cited By (since 1996)9

Cite this Research Publication

Ha Nagahama, Rani, V. V. Db, Shalumon, K. Tb, Jayakumar, Rab b, Nair, S. Vb, Koiwa, Sa, Furuike, Ta, and Tamura, Ha, “Preparation, characterization, bioactive and cell attachment studies of α-chitin/gelatin composite membranes”, International Journal of Biological Macromolecules, vol. 44, pp. 333-337, 2009.