Publication Type:

Journal Article

Source:

Journal of Proteome Research, Volume 11, Number 1, p.247-260 (2012)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84855533919&partnerID=40&md5=f691a5f28e4ee11d02f6a646e074a37e

Keywords:

amino acid sequence, article, Candida glabrata, codon, Fourier Analysis, fungal genome, fungal protein, Fungal Proteins, gene expression, gene identification, gene sequence, infrared spectroscopy, Initiator, Molecular Sequence Annotation, Molecular Sequence Data, nonhuman, nucleotide sequence, peptide, Peptide Fragments, peptide mapping, priority journal, protein analysis, proteome, proteomics, Reverse Transcriptase Polymerase Chain Reaction, reverse transcription polymerase chain reaction, tandem mass spectrometry

Abstract:

Candida glabrata is a common opportunistic human pathogen leading to significant mortality in immunosuppressed and immunodeficient individuals. We carried out proteomic analysis of C. glabrata using high resolution Fourier transform mass spectrometry with MS resolution of 60000 and MS/MS resolution of 7500. On the basis of 32453 unique peptides identified from 118815 peptide-spectrum matches, we validated 4421 of the 5283 predicted protein-coding genes (83%) in the C. glabrata genome. Further, searching the tandem mass spectra against a six frame translated genome database of C. glabrata resulted in identification of 11 novel protein coding genes and correction of gene boundaries for 14 predicted gene models. A subset of novel protein-coding genes and corrected gene models were validated at the transcript level by RT-PCR and sequencing. Our study illustrates how proteogenomic analysis enabled by high resolution mass spectrometry can enrich genome annotation and should be an integral part of ongoing genome sequencing and annotation efforts. © 2011 American Chemical Society.

Notes:

cited By (since 1996)4

Cite this Research Publication

T. S. Kab c d Prasad, Harsha, H. Ca, Keerthikumar, Sak, Sekhar, N. Rab, Selvan, L. D. Nad, Kumar, Pad, Pinto, S. Mac, Muthusamy, Bab, Subbannayya, Yae, Renuse, Sad g h, Chaerkady, Rag h, Mathur, P. Pb, Ravikumar, Rf, and Pandey, Agh i j, “Proteogenomic analysis of Candida glabrata using high resolution mass spectrometry”, Journal of Proteome Research, vol. 11, pp. 247-260, 2012.