Publication Type:

Journal Article

Source:

Pakistan Journal of Biological Sciences, Volume 14, Number 16, p.788-797 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-82255162082&partnerID=40&md5=8f1974e742b44ad8f832f236cc20928a

Keywords:

animal, Animals, antifungal agent, Antifungal Agents, article, Biological, biological pest control, chitinase, drug effect, enzyme inhibitor, Enzyme Inhibitors, enzyme specificity, enzyme stability, enzymology, Fungi, fungus, Hydrogen-Ion Concentration, isolation and purification, metabolism, Pest Control, pH, Streptomyces griseus, Substrate Specificity, temperature

Abstract:

Streptomyces griseus (MTCC 9723) is a chitinolytic bacterium isolated from prawn cultivated pond soil of Peddapuram Village; East Godavari District was studied in detailed. Chitinase (EC 3.2.1.14) was extracted from the culture filtrate of Streptomyces griseus and purified by ammonium sulfate precipitation, DEAE-cellulose ionexchange chromatography, Sephadex G-100 and Sephadex G-200 gel filtration chromatography. The molecular mass of the purified chitinase was estimated to be 34, 32 kDa by SDS gel electrophoresis and confirmed by activity staining with Calcofluor White M2R. Chitinase was optimally active at pH of 6.0 and at 40°C. The enzyme was stable from pH 5-9 and up to 20-50°C. The chitinase exhibited Km and Vmax values of 400 mg and 180 IU mL -1 for colloidal chitin. Among the metals and inhibitors that were tested, the Hg +, Hg 2+ and P-chloromercuribenzoic acid completely inhibited the chitinase activity at 1 mM concentration. The purified chitinase showed high activity on colloidal chitin, chitodiose, and chitooligosaccharide. An in vitro assay proved that the crude chitinase, actively growing cells of S. griseus having antifungal activity against all studied fungal pathogen. This result implies that characteristics of S. griseus producing endochitinase made them suitable for biotechnological purpose such as for degradation of chitin containing waste and it might be a promising biocontrol agent for plant pathogens. © 2011 Asian Network for Scientific Information.

Notes:

cited By (since 1996)3

Cite this Research Publication

Ma Rabeeth, Anitha, Ab, and Srikanth, Gc, “Purification of an antifungal endochitinase from a potential biocontrol agent Streptomyces griseus”, Pakistan Journal of Biological Sciences, vol. 14, pp. 788-797, 2011.