Publication Type:

Journal Article

Source:

Cancer Biology and Therapy, Volume 12, Number 6, p.510-522 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-80052855504&partnerID=40&md5=dd1f45c7923602bee261752943771eba

Keywords:

adult, aged, article, Biological, biological marker, cancer patient, cancer tissue, Carcinoma, clinical article, controlled study, Early Detection of Cancer, Esophageal Neoplasms, esophageal squamous cell carcinoma, heat shock protein 70, human, human cell, human tissue, Humans, immunohistochemistry, ion exchange chromatography, liquid chromatography, mass spectrometer, mass spectrometry, Plectin, prosaposin, protein analysis, Protein Disulfide-Isomerases, protein expression, proteome, proteomics, quantitative analysis, Saposins, Squamous Cell, tandem mass spectrometry, thrombospondin 1, tissue microarray, tumor marker, Tumor Markers, upregulation, validation process, validity

Abstract:

Esophageal squamous cell carcinoma (ESCC) is among the top ten most frequent malignancies worldwide. In this study, our objective was to identify potential biomarkers for ESCC through a quantitative proteomic approach using the isobaric tags for relative and absolute quantitation (iTRaQ) approach. We compared the protein expression profiles of ESCC tumor tissues with the corresponding adjacent normal tissue from ten patients. LC-MS/MS analysis of strong cation exchange chromatography fractions was performed on an accurate Mass QTOF mass spectrometer, which led to the identification of 687 proteins. In all, 257 proteins were identified as differentially expressed in ESCC as compared with normal. We found several previously known protein biomarkers to be upregulated in ESCC including thrombospondin 1 (THBS1), periostin 1 (POSTN) and heat shock 70 kDa protein 9 (HSPA9) confirming the validity of our approach. In addition, several novel proteins that had not been reported previously were identified in our screen. These novel biomarker candidates included prosaposin (PSAP), plectin 1 (PLEC1) and protein disulfide isomerase a 4 (PDIA4) that were further validated to be overexpressed by immunohistochemical labeling using tissue microarrays. The success of our study shows that this mass spectrometric strategy can be applied to cancers in general to develop a panel of candidate biomarkers, which can then be validated by other techniques.

Notes:

cited By (since 1996)13

Cite this Research Publication

Hab c Pawar, Kashyap, M. Ka, Sahasrabuddhe, N. Aag j k, Renuse, Sah j k, Harsha, H. Ca, Kumar, Pa, Sharma, Jag, Kandasamy, Kan, Marimuthu, Aag, Nair, Bh, Rajagopalan, Sd, Maharudraiah, Jae, Premalatha, C. Sc, Kumar, K. V. Vf, Vijayakumar, Mf, Chaerkady, Raj k, Prasad, T. S. Kag i, Kumar, R. Vc, and Pandey, Ajk l m, “Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery”, Cancer Biology and Therapy, vol. 12, pp. 510-522, 2011.