Publication Type:

Journal Article

Source:

Tissue Engineering - Part A, Mary Ann Liebert Inc., Volume 16, Number 2, p.393-404 (2010)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-77049107428&partnerID=40&md5=c027746caee2d48915517d306f54eba7

Keywords:

adsorption, alkaline phosphatase, animal cell culture, article, biological marker, Biological Markers, Blood Proteins, Bone, Bone and Bones, bone development, bone mineralization, Bone tissue engineering, Calcification, cell adhesion, Cell culture, cell motion, Cell Movement, cell proliferation, cell separation, cell shape, Cell Survival, Cells, chemistry, Cultured, Cytology, drug effect, Effect of In, Electrospuns, enzymology, Fiber surface, gene expression, Gene Expression Regulation, genetics, human, Human mesenchymal stem cells, Human mesenchymal stem cells (hMSCs), Humans, In-vitro, In-vivo, kinetics, Matrix mineralization, mesenchymal stem cell, Mesenchymal Stem Cells, metabolism, methodology, Microfibers, Microfibrous, nanofiber, Nanofibers, Nanofibrous scaffolds, Osteogenesis, Osteogenic, Osteogenic differentiation, Per unit, Physiologic, physiology, plasma protein, polycaprolactone, polyester, Polyesters, Protein surface, Proteins, Scaffolds, Size scale

Abstract:

In this study, we evaluated the role of fiber size scale in the adhesion and spreading potential of human mesenchymal stem cells (hMSCs) on electrospun poly(caprolactone) (PCL) nanofibrous and microfibrous scaffolds. The effect of in vivo regulators in inducing osteogenic differentiation of hMSCs on PCL nanofibrous scaffolds was investigated using osteogenic differentiation marker gene expression and matrix mineralization. Here, we report for the first time the influence of in vivo regulators in an in vitro setting with hMSCs for bone tissue engineering on PCL nanofibrous matrices. Our results indicated that hMSCs attached and spread rapidly on nanofibrous scaffolds in comparison to microfibrous PCL. Further, hMSCs proliferated well on the nanofibrous scaffolds. The cells on the nanofibrous PCL were found to differentiate into the osteoblast lineage and subsequently mineralize upon addition of in vivo osteogenic regulators. The attachment and spreading of hMSCs were more effective on the nanofibers compared with the microfibers despite the lower protein surface coverage (total adsorbed protein per unit fiber surface area) on nanofibers. Copyright 2010, Mary Ann Liebert, Inc.

Notes:

cited By 57

Cite this Research Publication

N. S. Binulal, Deepthy, M., Selvamurugan, N., Shalumon, K. T., Suja, S., Ullas Mony, Jayakumar, R., and Nair, S. V., “Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering-response to osteogenic regulators”, Tissue Engineering - Part A, vol. 16, pp. 393-404, 2010.