Publication Type:

Journal Article


Journal of Materials Science: Materials in Medicine, Volume 20, Number SUPPL. 1, p.S235-S241 (2009)



Anti-bacterial activity, Anti-Bacterial Agents, antibacterial activity, antineoplastic activity, Antineoplastic Agents, Aspect ratio, Bacteria, bacteriology, Biocidal action, Bone Neoplasms, cancer cell culture, Cancer cell lines, Cancer cells, Cell culture, Cells, conference paper, Controlled size, controlled study, Cultured, cytotoxicity, Cytotoxins, drug synthesis, Escherichia coli, Eukaryota, eukaryotic cell, Eukaryotic cells, Gram-negative bacteria, Gram-positive bacterium, human, Human cancer cells, human cell, Human osteoblast, Humans, Mammalia, Mammalian cells, Mammals, membrane, Micro-particles, Microbial Sensitivity Tests, Microspheres, Morphology, nanoparticle, Nanoparticle toxicity, Nanoparticles, Negibacteria, nonhuman, osteoblast, Osteoblasts, particle size, Polyethylene glycols, Posibacteria, Primary cells, priority journal, Prokaryota, prokaryotic cell, scanning electron microscopy, SEM, Size scale, Staphylococcus aureus, starch


The specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and morphology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia coli) as well as using Gram-positive bacteria (Staphylococcus aureus). Scanning electron microscopy (SEM) was conducted to characterize any visual features of the biocidal action of ZnO. We observed that antibacterial activity increased with reduction in particle size. Toxicity toward the human cancer cell line was considerably higher than previously observed by other researchers on the corresponding primary cells, suggesting selective toxicity of the ZnO to cancer cells. Surface capping was also found to profoundly influence the toxicity of ZnO nanoparticles toward the cancer cell line, with the toxicity of starch-capped ZnO being the lowest. Our results are found to be consistent with a membrane-related mechanism for nanoparticle toxicity toward microbes. © 2008 Springer Science+Business Media, LLC.


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Cite this Research Publication

Shantikumar V. Nair, Sasidharan, A., Rani, V. V. Divya, Menon, D., Raina, S., and Manzoor, K., “Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells”, Journal of Materials Science: Materials in Medicine, vol. 20, pp. S235-S241, 2009.