Secondary immunization inhibits drug induced apoptosis in vivo
Publication Type:Journal Article
Source:Indian Journal of Clinical Biochemistry, Volume 18, Number 2, p.161-168 (2003)
Keywords:animal experiment, animal tissue, Animalia, antibody response, apoptosis, article, cell proliferation, controlled study, drug effect, drug response, etoposide, immunization, lymphocyte, methotrexate, mouse, nonhuman, paraffin, spleen cell, tetanus toxoid, vincristine
Immunization with a proper dose of an antigenic stimulus leads to cell proliferation and antibody response of circulating lymphocytes. We have previously observed that Secondary immunized spleenocytes resist ceramide-mediated apoptosis in vitro. Our present study is aimed at investigating the in vivo effect of immunization on apoptosis. Mice were subjected to either Primary or Secondary dose with Tetanus Toxoid. Unimmunized spleenocytes served as controls. Unimmunized, Primary and Secondary immunized mice were later exposed to chemotherapeutic drugs such as Etoposide/Methotrexate/Vincristine to induce apoptosis. Apoptosis was studied by using the Feulgen reaction on 5μ thin parafin sections of spleen. It was observed that Secondary immunized mice showed a lower percentage of apoptosis as compared to Primary or Unimmunized mice that was subjected to either of the chemotherapeutic drugs. It was thus concluded that Secondary immunization inhibits the process of chemotherapeutic drug induced apoptosis in vivo.
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