Publication Type:

Journal Article

Source:

Arabian Journal of Chemistry, Volume 4, Number 2, p.163-168 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-79952040992&partnerID=40&md5=f97de1e9f5ba843bef5699911678006f

Keywords:

Acetonitrile, Body fluids, Concentration ranges, Electrospray ionization, extraction, Human urine, Ionization, Ionization sources, LC-MS, LC-MS-MS, liquid chromatography, Liquids, Lower limit of quantitations, Mass spectrometers, mass spectrometry, Mobile phase, Pantoprazole, Positive electrospray ionization, Quantification, Relative errors, Relative standard deviations, Sensitive liquid chromatography-tandem mass spectrometry, Sodium, Solid-phase extraction, Tandem mass spectrometric, Triple quadrupole, Urine sample

Abstract:

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine pantoprazole sodium (PNT) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C 18 column (symmetry 3.5μm; 75mm×4.6mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (90:10, v/v). The method was linear over a concentration range of 1-100ngmL -1. The lower limit of quantitation was 1ngmL -1. The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <10.5%. The accuracy determined at three concentrations (8.0, 50.0 and 85.0ngmL -1 PNT) was within ±1.25% in terms of relative errors. © 2010.

Notes:

cited By (since 1996)0

Cite this Research Publication

B. La Bhaskara, Kumar, U. RbAnil, and Basavaiah, Kb, “Sensitive liquid chromatography-tandem mass spectrometry method for the determination of pantoprazole sodium in human urine”, Arabian Journal of Chemistry, vol. 4, pp. 163-168, 2011.