Publication Type:

Journal Article


Asian Journal of Pharmaceutical and Clinical Research, Innovare Academics Sciences Pvt. Ltd, Volume 10, Number 7, p.204-206 (2017)



Objective: Spectrophotometric investigation of 4-nitro-2-phenoxyaniline (4N2PA), a probable intermediate during the synthesis of anti-inflammatory drug nimesulide, both in pure form and in the presence of nimesulide has been attempted. Methods: 4N2PA on diazotization followed by coupling with 8-hydroxyquinoline produces a crimson-colored complex which changes to deep violet on diluting with ethanol. Results: The complex showed maximum absorbance at 560 nm when evaluated spectrophotometrically with a detection limit of 0.005 µg/ml and quantification range of 0.05-3.0 µg/ml. The method has been statistically evaluated with respect to the International Council for Harmonisation guidelines and found to be accurate and precise. Conclusion: Pure tablet formulations of nimesulide do not respond to the method; however, the presence of minute amounts of 4N2PA in the drug as added impurity could be spectrophotometrically analyzed. Hence, the authors suggest that the reported technique could be a marker test for detecting the presence of 4N2PA in nimesulide formulations. © 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd.


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Cite this Research Publication

S. Anil Kumar and B.L. Bhaskar, “Spectrophotometric evaluation of nimesulide impurity d: 4-nitro-2-phenoxyaniline using 8-hydroxyquinoline as oxidative coupling reagent”, Asian Journal of Pharmaceutical and Clinical Research, vol. 10, pp. 204-206, 2017.