Structural Basis of Guanine Nucleotide Exchange Mediated by the T-Cell Essential Vav1
Publication Type:Journal Article
Source:Journal of Molecular Biology, Volume 380, Number 5, p.828-843 (2008)
Keywords:alanine, Amino Acid, amino acid sequence, amino acid substitution, article, carboxy terminal sequence, crystal structure, crystallography, enzyme activation, guanine nucleotide exchange factor, Guanine Nucleotide Exchange Factors, human, human cell, Humans, Hydrogen Bonding, Models, Molecular, Molecular Sequence Data, molecular weight, mutational analysis, priority journal, Protein Binding, protein conformation, protein expression, Protein interaction, protein protein interaction, protein stability, Protein Structure, protein vav1, Proto-Oncogene Proteins c-vav, rac1 GTP-Binding Protein, Rac1 protein, Recombinant Proteins, rho GTP-Binding Proteins, Rho guanine nucleotide binding protein, Scattering, Secondary, sequence homology, Small Angle, solubility, T lymphocyte activation, T-Lymphocytes, Tertiary, X ray crystallography, X-Ray, X-Ray Diffraction
The guanine nucleotide exchange factor (GEF) Vav1 plays an important role in T-cell activation and tumorigenesis. In the GEF superfamily, Vav1 has the ability to interact with multiple families of Rho GTPases. The structure of the Vav1 DH-PH-CRD/Rac1 complex to 2.6 Å resolution reveals a unique intramolecular network of contacts between the Vav1 cysteine-rich domain (CRD) and the C-terminal helix of the Vav1 Dbl homology (DH) domain. These unique interactions stabilize the Vav1 DH domain for its intimate association with the Switch II region of Rac1 that is critical for the displacement of the guanine nucleotide. Small angle x-ray scattering (SAXS) studies support this domain arrangement for the complex in solution. Further, mutational analyses confirms that the atypical CRD is critical for maintaining both optimal guanine nucleotide exchange activity and broader specificity of Vav family GEFs. Taken together, the data outline the detailed nature of Vav1's ability to contact a range of Rho GTPases using a novel protein-protein interaction network. © 2008 Elsevier Ltd. All rights reserved.
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