Publication Type:

Journal Article

Source:

Biochimica et Biophysica Acta - Proteins and Proteomics, Volume 1804, Number 2, p.245-262 (2010)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-74649085703&partnerID=40&md5=bb962eca59cb306aaa821a887bdfba63

Keywords:

Amino Acid, amino acid sequence, amyotrophic lateral sclerosis, Animals, Biochemistry, biology, catalysis, clinical research, comprehension, copper zinc superoxide dismutase, crystal structure, Diffusion, dimer, disulfide, disulfide bond, Electricity, enzyme active site, enzyme activity, enzyme mechanism, enzyme structure, fiber, human, Humans, Hydrogen, hydrogen bond, iron superoxide dismutase, ligand, manganese superoxide dismutase, metal, metal binding, Molecular Sequence Data, Mutation, mutational analysis, negative feedback, nerve degeneration, nickel superoxide dismutase, nonhuman, oxidation reduction potential, pathogenesis, phosphate, priority journal, protein motif, review, sequence homology, superoxide dismutase, unclassified drug

Abstract:

The discovery of superoxide dismutases (SODs), which convert superoxide radicals to molecular oxygen and hydrogen peroxide, has been termed the most important discovery of modern biology never to win a Nobel Prize. Here, we review the reasons this discovery has been underappreciated, as well as discuss the robust results supporting its premier biological importance and utility for current research. We highlight our understanding of SOD function gained through structural biology analyses, which reveal important hydrogen-bonding schemes and metal-binding motifs. These structural features create remarkable enzymes that promote catalysis at faster than diffusion-limited rates by using electrostatic guidance. These architectures additionally alter the redox potential of the active site metal center to a range suitable for the superoxide disproportionation reaction and protect against inhibition of catalysis by molecules such as phosphate. SOD structures may also control their enzymatic activity through product inhibition; manipulation of these product inhibition levels has the potential to generate therapeutic forms of SOD. Markedly, structural destabilization of the SOD architecture can lead to disease, as mutations in Cu,ZnSOD may result in familial amyotrophic lateral sclerosis, a relatively common, rapidly progressing and fatal neurodegenerative disorder. We describe our current understanding of how these Cu,ZnSOD mutations may lead to aggregation/fibril formation, as a detailed understanding of these mechanisms provides new avenues for the development of therapeutics against this so far untreatable neurodegenerative pathology. © 2009 Elsevier B.V. All rights reserved.

Notes:

cited By (since 1996)51

Cite this Research Publication

J. J. Pab Perry, Shin, D. Sa, Getzoff, E. Da, and Tainer, J. Aac, “The structural biochemistry of the superoxide dismutases”, Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1804, pp. 245-262, 2010.