Publication Type:

Journal Article


Current Opinion in Structural Biology, Volume 20, Number 3, p.283-294 (2010)



6 o alkylguanine DNA alkyltransferase, Animals, crystallography, DNA, DNA cross linking, DNA damage, DNA dependent protein kinase, DNA polymerase, DNA protein complex, DNA repair, DNA Replication, DNA sequence, DNA structure, double stranded DNA break, enzyme activity, Enzymes, excision repair, Humans, molecular dynamics, nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1, nuclear magnetic resonance, polydeoxyribonucleotide synthase, polynucleotide 5' hydroxyl kinase, priority journal, protein DNA binding, protein domain, protein function, Proteins, review


Changing macromolecular conformations and complexes are critical features of cellular networks, typified by DNA damage response pathways that are essential to life. These fluctuations enhance the specificity of macromolecular recognition and catalysis, and enable an integrated functioning of pathway components, ensuring efficiency while reducing off pathway reactions. Such dynamic complexes challenge classical detailed structural analyses, so their characterizations demand combining methods that provide detail with those that inform dynamics in solution. Small-angle X-ray scattering, electron microscopy, hydrogen-deuterium exchange and computation are complementing detailed structures from crystallography and NMR to provide comprehensive models for DNA damage searching, specificity, signaling, and repair. Here, we review new approaches and results on DNA damage responses that advance structural biology in the fourth dimension, connecting proteins to pathways. © 2010 Elsevier Ltd.


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Cite this Research Publication

J. J. Pab Perry, Cotner-Gohara, Ec, Ellenberger, Tc, and Tainer, J. Aad, “Structural dynamics in DNA damage signaling and repair”, Current Opinion in Structural Biology, vol. 20, pp. 283-294, 2010.