Publication Type:

Journal Article


Journal of Biomedical Nanotechnology, Volume 8, Number 1, p.149-160 (2012)



adsorption, article, biocompatibility, Biomaterials, Biomechanics, biomineralization, Blood Proteins, Bone, Bone and Bones, Bone regeneration, bone tissue, Bone tissue engineering, Calcification, cell adhesion, Cell attachments, Cell Line, cell proliferation, Cell seeding, Cells, chitosan, chondroitin sulfate, Chondroitin sulfates, Composite scaffolds, controlled study, Cytology, degradation, Fibrin, freeze drying, Glycosaminoglycans, human, human cell, Humans, In-vitro, Mechanical integrity, Mechanical properties, Nano- SiO, nanocomposite, Nanocomposites, nanofabrication, Osteoconductive, Physiologic, porosity, Protein adsorption, Scaffolds (biology), silicon dioxide, synthesis, tissue engineering, Tissue Scaffolds, Tumor


Chitosan, a natural polymer, is a biomaterial which is known to be osteoconductive but lacking in mechanical strength. In this work, to further enhance the mechanical property and biocompatibility of chitosan, we combined it with both chondroitin sulfate, a natural glycosaminoglycan found in bone, and nano-SiO 2. The composite scaffold of chitosan/chondroitin sulfate/nano-SiO 2 was fabricated by lyophilization. The nanocomposite scaffold showed enhanced porosity, degradation, mechanical integrity, biomineralization and protein adsorption. Biocompatibility and cell attachment-proliferation studies performed using MG-63 cells, advocate its better performance in vitro. To improve the cell seeding efficiency, we coated the scaffold surface with fibrin, which enhanced the initial cell attachment. The cumulative results suggest this novel nanocomposite scaffold to be a suitable candidate for bone tissue engineering. Copyright © 2012 American Scientific Publishers All rights reserved.


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Cite this Research Publication

K. C. Kavya, Dixit, R., Jayakumar, R., Nair, S. V., and Chennazhi, K. P., “Synthesis and characterization of chitosan/chondroitin sulfate/nano- SiO 2 composite scaffold for bone tissue engineering”, Journal of Biomedical Nanotechnology, vol. 8, pp. 149-160, 2012.