Human Parathyroid hormone 1-34 (PTH1-34) loaded chitosan nanoparticles (PTH 1-34 chitosan nanoparticles) via simple ionic gelation technique were prepared which can improve the bioavailability and half-life of the peptide. Chitosan nanoparticles and PTH 1-34 chitosan nanoparticles were synthesised and characterized by DLS, SEM, AFM, FT-IR and TG/DTA. Chitosan nanoparticles (40-60 nm) and PTH 1-34 chitosan nanoparticles (60-80 nm) with zeta potential of +60 and +40 mV respectively were subjected to haemolysis assay and tested for agglomeration in blood. MTT and LDH was performed assay using Saos-2, UMR 106, L929, NIH3T3. The in vitro peptide release profile at pH 7.5 for 144 h was quantified using PTH 1-34 ELISA Kit. Effect of released PTH 1-34 on Saos-2 was determined with ALP and BCA assay. These preliminary results pave way for the prospective use of such a carrier for the delivery of PTH 1-34 by multiple routes for the benefit of patients undergoing treatment for osteoporosis. Copyright © 2012 American Scientific Publishers All rights reserved.
cited By 15
D. Narayanan, Anitha, A., Dr. Jayakumar Rangasamy, Nair, S. V., and Chennazhi, K. P., “Synthesis, characterization and preliminary in vitro evaluation of PTH 1-34 loaded chitosan nanoparticles for osteoporosis”, Journal of Biomedical Nanotechnology, vol. 8, pp. 98-106, 2012.