We have developed a novel, operator-friendly, nanothermite reaction actuator which generates transient pressures comprised of a shock wave superimposed on a broad pressure pulse to facilitate intranuclear molecular transport. The actuator demonstrates the delivery of ∼70 kDa FITC-Dextran into chicken cardiomyocytes with cytoplasmic delivery efficiency greater than 90%, maximum intranuclear delivery efficiency of 84%, and cell survival rates exceeding 95% in minimum operating pressure conditions. Tunable nanothermite reactions enable versatile pressure generating characteristics which can extend the technology to a spectrum of biomedical molecular delivery applications.
M. Korampally, Apperson, S. J., Staley, C. S., Castorena, J. A., Dr. T. Rajagopalan, Gangopadhyay, K., Mohan, R. R., Ghosh, A., Polo-Parada, L., and Gangopadhyay, S., “Transient pressure mediated intranuclear delivery of FITC-Dextran into chicken cardiomyocytes by MEMS-based nanothermite reaction actuator”, Sensors and Actuators B: Chemical, vol. 171–172, pp. 1292 - 1296, 2012.