Publication Type:

Journal Article

Source:

Colloids Surf B Biointerfaces, Volume 159, p.809-819 (2017)

Keywords:

curcumin, Drug Liberation, Humans, Hydrogen-Ion Concentration, Nanoparticles, polyglutamic acid, Polymers, Spectrometry, Mass, Electrospray Ionization, tumor microenvironment

Abstract:

<p>Tunable pH and redox responsive polymer was prepared using γ-polyglutamic acid (γ-PGA) with linker 3-mercaptopropionic acid (3-MPA) (γ-PGA_SH) via oxidation to obtain redox responsive disulfide (γ-PGA_SS) backbone and adipic acid dihydrazide (ADH) (γ-PGA_SS_ADH) with hydrazide functional group for pH responsiveness. Further curcumin (Cur) was conjugated through hydrazone bond of the γ-PGA_SS_ADH via Schiff base reaction to obtain (γ-PGA_SS_ADH_Cur). The prepared systems were characterized by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, Electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Qq-TOF-MS/MS) and Solid state nuclear magnetic resonance (SS NMR) techniques. γ-PGA_SS_ADH_Cur formed self-assembled core shell nanoparticles (NPs) in existence of stabilized aqueous medium. γ-PGA_SS_ADH_Cur NPs maintained its stability in physiological condition. NPs tunable Cur release and cytotoxicity were observed for γ-PGA_SS_ADH_Cur NPs in both acidic and redox conditions mimicking the cancer microenvironment. γ-PGA_SS_ADH_Cur NPs uptake study showed via endocytosis mechanism resulted in the lysosomal entrapment of these NPs within the cell. γ-PGA_SS_ADH_Cur NPs exhibited a dual stimuli responsive drug delivery and can be used as a smart and potential drug delivery system in cancer microenvironment.</p>

Cite this Research Publication

S. Pillarisetti, Maya, S., Sathianarayanan, S., and Dr. Jayakumar Rangasamy, “Tunable pH and Redox-responsive Drug Release from Curcumin Conjugated γ-polyglutamic Acid Nanoparticles in Cancer Microenvironment”, Colloids Surf B Biointerfaces, vol. 159, pp. 809-819, 2017.