Publication Type:

Journal Article

Source:

Sensors and Actuators, B: Chemical, Elsevier B.V., Volume 268, p.195-204 (2018)

URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046108273&doi=10.1016%2fj.snb.2018.04.092&partnerID=40&md5=1b876db195bf9f0b3eab4727f068766a

Keywords:

Alkalinity, Computer circuits, Excitation wavelength, Fluorescence, Internal calibration, Intracellular, Logic gates, Mitochondria, Nutrient-starvation, pH, pH meters, PH probe, Photons, Probes, Real time monitoring, Spectroscopic behavior, Two photon fluorescence

Abstract:

Mapping the intracellular location and concentration of hydronium ions (H3O+) and their dynamics could be a useful diagnostic tool in modern clinical science. Current needs motivated us to develop a molecular pH probe 1, operating as a logic gate, and its analogue 2. The pyridyl moiety in 1 played a significant role in proton capture and release, in acidic to alkaline pH environments. In contrast, 2 failed to show a similar spectroscopic behavior. 1 shows emission maximum at 450 nm that is independent on the pH, with excitation at 353 nm or 410 nm in acid and alkaline pH, respectively. 1 was employed to provide input-dependent (excitation wavelength) fluorescence images in a cellular milieu to detect pH changes in cellular organelles such as lysosomes and mitochondria. Furthermore, 1 provided information on the variation of the pH in the presence of cellular ROS. 1 was also found to enable the real-time monitoring of cell acidification due to nutrient starvation, which is closely associated with mitochondrial malfunction, fusion and mitophagy processes. We envision that in due course 1 can open up new research avenues in the diagnostic sector for validating the pH in the cellular milieu. © 2018 Elsevier B.V.

Notes:

cited By 0

Cite this Research Publication

A. Podder, Won, M., Kim, S., Verwilst, P., Maiti, M., Yang, Z., Qu, J., Bhuniya, S., and Kim, J. S., “A two-photon fluorescent probe records the intracellular pH through ‘OR’ logic operation via internal calibration”, Sensors and Actuators, B: Chemical, vol. 268, pp. 195-204, 2018.