Publication Type:

Journal Article


Life Sciences, Volume 88, Number 15-16, p.675-680 (2011)



1, 3 dicyanobutadiene, 3T3-L1 Cells, 4 bis(2 aminophenylthio) 2, 4 diamino 1, actin, adipocyte, Adipocytes, Animals, article, Benzaldehydes, cell differentiation, controlled study, Dose-Response Relationship, drug, enzyme activation, enzyme regulation, gene targeting, Glucose, glucose transporter 4, Glucose Transporter Type 4, insulin, insulin sensitivity, intracellular signaling, lipid, lipid analysis, lipid storage, Mice, mitogen activated protein kinase 42, mitogen activated protein kinase 44, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, mouse, Murinae, nonhuman, peroxisome proliferator activated receptor gamma, Phosphorylation, PPAR gamma, proadipocyte, protein expression, protein kinase, protein kinase B, protein kinase B beta, Proto-Oncogene Proteins c-akt, regulatory mechanism, signal transduction, unclassified drug, vanillin


Aims: To investigate the effect of vanillin, a dietary component, on adipocyte differentiation and the mechanism involved in the process using 3T3-L1 murine preadipocytes. Main methods: The effect of vanillin on adipocyte differentiation was detected by Oil Red O analysis. The activation of extracellular signal regulated kinase 42/44 (ERK 42/44), Akt, expression of the key regulator of adipocyte differentiation peroxisome proliferators-activated receptor (PPARγ) and its target gene glucose transporter 4 (GLUT4) were detected by western blotting. Glucose uptake assay was used to determine the insulin sensitivity of adipocytes differentiated by vanillin treatment. To confirm the role of ERK 42/44 and Akt, Oil Red O analysis was performed with cells differentiated in the presence or absence of ERK inhibitor U0126 or Akt kinase 1/2 inhibitor. Key findings: Vanillin induced adipocyte differentiation in 3T3-L1 cells in a dose dependent manner and also increased the expression levels of PPARγ and its target gene GLUT4. The adipocytes differentiated by vanillin exhibited insulin sensitivity as demonstrated by a significant increase in glucose uptake. Vanillin treatment activated the phosphorylation of ERK 42/44 during the initial phase of adipocyte differentiation but there was no significant change in the Akt phosphorylation status. Significance: The data show that vanillin induces adipocyte differentiation in 3T3-L1 cells by activating ERK42/44 and these adipocytes are insulin sensitive in nature. © 2011 Elsevier Inc.


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Cite this Research Publication

P. A. Haridas Nidhina, Poulose, N., and Gopalakrishnapillai, A., “Vanillin induces adipocyte differentiation in 3T3-L1 cells by activating extracellular signal regulated kinase 42/44”, Life Sciences, vol. 88, pp. 675-680, 2011.