Publication Type:

Journal Article


Colloids and Surfaces B: Biointerfaces, Volume 104, p.245-253 (2013)



Anticancer activities, antineoplastic activity, Antineoplastic Agents, Antitumor, apoptosis, article, Average diameter, biocompatibility, biodegradability, cell density, cell proliferation, Cell Survival, Cellular internalization, Chemotherapeutic agents, chitin, Colon cancer, Colon cancer cells, Colonic Neoplasms, conformational transition, controlled study, cross linking, Crosslinking, Crosslinking reaction, Cultured, cytotoxicity, deacetylation, Dose-Response Relationship, drug, Drug delivery, Drug delivery applications, drug delivery system, Drug Delivery Systems, Drug products, drug release, Drug Screening Assays, flow cytometry, fluorescence microscopy, Fluorescent microscopy, HT29 Cells, human, human cell, Humans, hydrogen bond, in vitro study, In-vitro, In-vitro evaluation, internalization, Ionic cross-linking, limit of detection, limit of quantitation, membrane permeability, nanoparticle, Nanoparticles, paclitaxel, particle size, Physico-chemical characterization, Phytogenic, priority journal, scanning electron microscopy, Structure-Activity Relationship, succinate dehydrogenase, Surface properties, Sustained release, Tumor Cells, validation process, X ray diffraction, zeta potential


Chitin and its derivatives have been widely used in drug delivery applications due to its biocompatible, biodegradable and non-toxic nature. In this study, we have developed amorphous chitin nanoparticles (150 ± 50. nm) and evaluated its potential as a drug delivery system. Paclitaxel (PTX), a major chemotherapeutic agent was loaded into amorphous chitin nanoparticles (AC NPs) through ionic cross-linking reaction using TPP. The prepared PTX loaded AC NPs had an average diameter of 200 ± 50. nm. Physico-chemical characterization of the prepared nanoparticles was carried out. These nanoparticles were proven to be hemocompatible and in vitro drug release studies showed a sustained release of PTX. Cellular internalization of the NPs was confirmed by fluorescent microscopy as well as by flow cytometry. Anticancer activity studies proved the toxicity of PTX-AC NPs toward colon cancer cells. These preliminary results indicate the potential of PTX-AC NPs in colon cancer drug delivery. © 2012 Elsevier B.V.


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Cite this Research Publication

, “In vitro evaluation of paclitaxel loaded amorphous chitin nanoparticles for colon cancer drug delivery”, Colloids and Surfaces B: Biointerfaces, vol. 104, pp. 245-253, 2013.