In vitro hemocompatibility and vascular endothelial cell functionality on titania nanostructures under static and dynamic conditions for improved coronary stenting applications
Publication Type:Journal Article
Source:Acta Biomaterialia, Volume 9, Number 12, p.9568-9577 (2013)
Keywords:artery blood flow, article, blood clotting, blood clotting time, blood compatibility, blood vessel reactivity, cell function, chemical modification, circulation, controlled study, coronary stent, endothelium, endothelium cell, gene expression, Hemolysis, human, human cell, inflammation, metal stent, microvascular endothelial cell, molecular interaction, nanomaterial, priority journal, thrombocyte activation, thrombocyte aggregation, Titanium dioxide, topography
The usefulness of nanoscale topography in improving vascular response in vitro was established previously on hydrothermally modified titanium surfaces. To propose this strategy of surface modification for translation onto clinically used metallic stents, it is imperative that the surface should be also hemocompatible: an essential attribute for any blood-contacting device. The present in vitro study focuses on a detailed hemocompatibility evaluation of titania nanostructures created through an alkaline hydrothermal route on metallic Ti stent prototypes. Direct interactions of TiO2 nanocues of various morphologies with whole blood were studied under static conditions as well as using an in vitro circulation model mimicking arterial flow, with respect to a polished Ti control. Nanomodified stent surfaces upon contact with human blood showed negligible hemolysis under constant shear and static conditions. Coagulation profile testing indicated that surface roughness of nanomodified stents induced no alterations in the normal clotting times, with insignificant thrombus formation and minimal inflammatory reaction. Endothelialized nanomodified Ti surfaces were found to inhibit both activation as well as aggregation of platelets compared with the control surface, with the endothelium formed on the nanosurfaces having an increased expression of anti-thrombogenic genes. Such a nanotextured Ti surface, which is anti-thrombogenic and promotes endothelialization, would be a cost-effective alternative to drug-eluting stents or polymer-coated stents for overcoming in-stent restenosis. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
cited By (since 1996)1
Cite this Research Publication
Related Research Publications
- Influence of titania nanotopography on human vascular cell functionality and its proliferation in vitro
- Hematotoxicological analysis of surface-modified and-unmodified chitosan nanoparticles
- Hemocompatibility and macrophage response of pristine and functionalized graphene.
- Hemocompatibility and macrophage response of pristine and functionalized graphene
- In vitro and in vivo evaluation of microporous chitosan hydrogel/nanofibrin composite bandage for skin tissue regeneration