It is well known that spices increases the nutritional value of food. In this study five different essential oils from spices such as Cardamom ,Clove, Garlic, Ginger and Cinnamon were test against E.coli. It was observed that Cardamom and Cinnamon shows highest activity. Ginger and Garlic oil showed no activity against the organism. Synergistic action of Cinnamon with Cardamom and ginger were also observed. Methanolic, Chloroform and petroleum ether extracts of Cardamom were also tested against E.coli.In that only methanolic extract shows antibacterial activity.