Diabetes mellitus is a public health problem affecting millions of people worldwide. Commercially available glucose sensors are enzyme based and has serious drawbacks like insufficient long-term stability, which originates from the intrinsic nature of the enzymes. This project aims at development and fabrication of non-enzymatic glucose sensor and a glucometer. Electrode surface modified with nanomaterials such as carbon nanotubes and transition metal nanoparticles have been used for the development of non-enzymatic sensors for the amperometric measurements of glucose. Sensors and biosensors modified with metal nanoparticles reveal good performances such as increased mass transport, catalysis, good biocompatibility and control over the electrode microenvironment when compared with macroelectrodes. Therefore, their use is an important strategy in the construction of non-enzymatic glucose sensors.