The present study is a comparison of the data of spectral analysis of heart rate variability with clinical evaluation of pathological state of dosas. The calculated cardiointervalography values are combined into three integral indexes, which according to the opinion of the investigators reflect the influence on heart rhythm of Vāta, Pitta and Kapha, the regulation systems of the body known as doṣas in Ayurveda. Seven gross doṣa imbalances were assessed to test the agreement between the two methods in this study. Heart Rate Variability Spectral Data was collected from 42 subjects to make the comparison with the clinical assessment of doṣa imbalance. Clinical method of doṣa assessment and method of calculating integral indexes by cardiointervalography data showed substantial agreement by Kappa Co-efficient statistic (k = 0.78) in assessment of gross doṣa imbalance. The results of the data generated from this pilot study warrants further studies to rigorously validate the algorithms of HRV analysis in understanding dosa imbalance in Ayurvedic clinical practice and research settings.