Fibronectin is one of the extraceullar proteins in vertebrates generated by alternative splicing, with twenty more possible isoforms. This project involves a survey of current fibronectin literature, followed by an account of our experiments to purify fibronectin from plasma using gelatin affinity chromatography. Gelatin was conjugated/copolymerized with agarose or polyacrcylamide and their efficacies of the purification were compared by SDS-PAGE followed by densitometric scanning and analysis by ImageJ software.

Project Details
PI: 
Sanjay Pal
Co Pi: 
Veena, Uma Maheswari, Suvitha, Archana Kini, Swathy Marrar, Arjun KV, Uma VK, Jyostna T, Lekshmi Sankar, Vignesh Narayan H
Start Date: 
Saturday, January 1, 2011 to Monday, May 30, 2011

School: