• Lack of drinking water and proper sanitation are the major causes of enteric infection leading to huge malnutrition and other health problems in developing nations. Solving the sanitation challenge in the developing world will require radically new innovations that are deployable on a large scale. We aim to reduce the bacterial load of waste water by developing bacteriophages from sewage against the enteric pathogens. Bacteriophages are the viruses which infect bacteria and kill them.

    characterization-of-the-bacteriophages-binding-with-human-matrix-proteins
  • Bacillus clausii, a gram positive, spore forming bacteria is widely used a probiotic in preventing and treating various gastrointestinal disorders, are known to colonize in host systems. But Staphylococcus aureus, though gram positive and show ability to colonize in host systems, is pathogenic in nature.

    isolation-and-characterization-of-host-binding-proteins-from-bacillus-clausii-and-staphylococcus-aureus-using-mass-spectrometry-a-proteomic-approach
  • Poor sanitation contributes to 1.5 million child deaths globally from diarrhoea alone caused by enteric pathogens each year. Solving the sanitation challenge in the developing world will require radically new innovations that are deployable on a large scale. Hence, we aimed to reduce the bacterial load of waste water by developing bacteriophages from sewage against the enteric pathogens. Bacteriophages are the viruses which infect bacteria and kill them.

    isolation-of-bacteriophages-against-enteric-pathogens-from-sewage
  • Aspergillus sp. has the ability to produce Naringinase enzyme in a selective media under specific conditions. Naringinase has α-L-rhamnosidase and β-D-glucosidase activities. This hydrolytic enzymatic complex has wide occurrence in nature and has been reported in plants, yeasts, fungi and bacteria. Naringinase is commercially attractive due to its potential usefulness in pharmaceutical and food industries.

    production-purification-and-application-of-naringinase-from-soil-isolates-of-aspergillus-sp
  • Peptidoglycan (PG) hydrolases or autolysins are a group of enzymes which degrade bacterial cell wall at specific sites. Staphylococcus aureus produces two major PG hydrolases: major autolysin (Atl) and Aaa, a autolysin/adhesin protein. Amidase are surface-associated proteins that have both enzymatic and adhesive functions.

    expression-purification-and-refolding-of-recombinant-amidase-in-escherichia-coli
  • The folding of DNA to create nanoscale shapes is known more commonly as DNA origami. DNA origami is a fairly new technique in field of nanobiotechnology. It is mainly used in drug target transfer process and drug delivery. It is also used in making fairly simple designs such as smileys, dolphins, trapezium square etc. This again used to create three dimensional structures too for example cube, capsules etc.

    folding-of-the-vector-pcdh-cmv-mcs-ef1-puro-into-predefined-shape-using-20mer-staples
  • Microflora of fish intestines appear to vary with the complexity of the fish digestive system. The genera present in the gut generally seem to be those from the environment or diet which can survive and multiply in the intestinal tract. The main aim of the work is to identify the organism present in the gut of fish through ribotyping technique. To start up with the procedure first standardized the ribotyping technique.

    ribotyping-of-fish-microflora
  • Peptidoglycan (PG) hydrolases or autolysins are a group of enzymes which degrades bacterial cell wall at specific sites. Staphylococcus aureus produces two major PG hydrolases: major autolysin (AtlA) and Aaa, a autolysin/adhesin protein. Amidase are surface-associated proteins that have both enzymatic and adhesive functions. AtlA is the most predominant autolysin in Staphylococcus aureus. The AltA protein only with an amidase domain and the two repeat sequences R1 and R2 is 62 kDa.

    expression-and-refolding-of-recombinant-staphylococcal-amidases-in-e-coli
  • DNA microarrays have rapidly evolved to become one of the essential tools to examine expression or mutation of thousands of genes simultaneously. There is a need for alternative patterning methods that must be very simple, reproducible, cost-effective, and eventually transferable to any laboratories for their own problematic situations.

    benchtop-nanoscale-patterning-using-soft-lithography-for-the-printing-of-dna-molecules
  • India is rich in plant biodiversity, and that has been utilized extensively in traditional medicine. In collaboration with Dr. Asoke Banerji (Phytochemistry, Amrita School of Biotechnology) who is leading the efforts in the extraction and purification of natural products from various plant sources, we plan to test these compounds on the expression of gelatinases (MMP-2 and MMP-9); known to be up-regulated in majority of cancers including breast and colon cancers.

    modulation-of-microrna-and-transcriptional-regulation-of-mmp-2-by-natural-products-in-human-breast-cancer-cells
  • MicroRNAs (miRNAs) are short double stranded RNAs that are produced endogenously in mammalian cells, and control the regulation of many target genes at the post-transcriptional level. There are many reports of the misregulation of these miRNAs in cancer. We will be studying the regulation of MMP-2 & MMP-9 by miRNAs, and the effect of natural products on the modulation of these miRNAs.

    the-discovery-of-micrornas-mirnas-that-regulate-the-expression-of-gelatinase-a-matrix-metalloproteinase-2-mmp-2-and-b-matrix-metalloproteinase-9-mmp-9-in-colon-cancer-cells
  • Investigating the organized activity of neuron ensembles is critical to understand how brain circuits work and is one main target of the project. Reconstruction of network activity has proved to be a hard task. This project will study cellular function using multi-scale models of single cells and networks will be developed with NEURON.

    computational-modelling-and-prediction-of-cerebellar-input-layer-function-timing-and-plasticity-for-understanding-neurophysiological-disorders

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