• Fibronectin, an extra cellular matrix glycoprotein was purified from human plasma obtained from Regional Cancer Centre Trivandrum. Gelatin based affinity chromatography was used for the purification of the protein. The usage of pressure was found to have prominent impact in the purification of the protein by reducing albumin contamination, which we suspect would have non specifically bound to the gelatin resin. The quality of the protein purified was analysed using SDS –PAGE.

    fibronectin-purification-and-fragmentation
  • As the importance of blood glucose control for both diabetic and non diabetic patients continues to increase, there is a need for advanced glucose sensing technologies. The low accuracy and sensor drift become major problems with in vivo environments especially for enzyme based electrochemical glucose sensors. This study dealt with the use of E.coli as an effective biorecognition element in a whole cell based biosensor. A lac operon based sensor has been decided to develop.

    studies-on-the-use-of-bacterial-cell-as-glucose-biosensor
  • The list of pollutants which pose environmental and health hazard are tough for biodegradation, is a long one and involves solvents, wood preservative chemicals, refrigerants, plasticizers, coal tar wastes, pesticides, biphenyls, synthetic fibers, plastics etc. The present study deals with the isolation of plastic degrading microorganisms from the plastic samples, analysis of physical or changes produced during biodegradation and the study of enzymes involved in biodegradation.

    biodegradation-of-plastics-using-fungal-isolates
  • Peptidoglycan (PG) hydrolases or autolysins are a group of enzymes which degrades bacterial cell wall at specific sites. Staphylococcus aureus produces two major PG hydrolases: major autolysin (AtlA) and Aaa, a autolysin/adhesin protein. Amidase are surface-associated proteins that have both enzymatic and adhesive functions. AtlA is the most predominant autolysin in Staphylococcus aureus. The AltA protein only with an amidase domain and the two repeat sequences R1 and R2 is 62 kDa.

    expression-and-refolding-of-recombinant-staphylococcal-amidases-in-e-coli
  • The current studies provided a direct illustration that electrical power can be generated economically in a two chambered microbial fuel cell. Under the anaerobic condition at the anode, the electrons released by the Proteus vulgaris are directed towards the cathodic chamber through an external circuit. Low cost and less oligodynamicity encouraged the use aluminium as anode. Anaerobic condition of the anodic chamber is achieved by using an air-tight rubber suction outer lid.

    design-construction-and-operation-of-a-prototypes-of-phototrophic-microbial-fuel-cells
  • This project proposes to develop a cerebellum inspired pattern recognition algorithm for robotic data classification. The project aims to investigate the temporal and spatial dynamics in the cerebellar network models capable of predicting cerebellar input-output transformations by analyzing the mathematical and computational properties of the network. Both labs have been working together since 2004 on cerebellar models.

    cerebellum-inspired-approach-for-pattern-classification-in-robots
  • Bacillus clausii, a gram positive, spore forming bacteria is widely used a probiotic in preventing and treating various gastrointestinal disorders. In order to understand the functioning of this strain as a probiotic, one needs to study the molecular level of interaction of this strain with host systems. Considering the fact that the first level of interaction can happen at the extracellular level, the project aims to characterize extracellular matrix proteins of Bacillus calusii.

    isolation-and-characterization-of-host-binding-proteins-from-bacillus-clausii-using-mass-spectrometry-a-proteomic-approach
  • The project titled "Lab-on-a-chip(LOC) for the monitoring of diabetes, cholesterol and kidney function" is funded by DBT, Govt. of India. Dr. Bipin Nair is the Principal Investigator of the project.

    lab-on-a-chip-loc-for-the-monitoring-of-diabetes-cholesterol-and-kidney-function
  • There are reports on derivatives of azobenzene that exhibit light-triggered association of Bovine Serum Albumin. To this end, an azobenzene-triazine conjugate was prepared by reacting Disperse Orange 3 dye with trichlorotrizaine. This intermediate was then conjugated to the biopolymer chitosan by a facile nucleophilic substitution.

    photo-switchable-azobenzene-conjugated-matrices-for-affinity-chromatography-synthesis-characterization-and-studies
  • Flavanoids like naringin and naringenin are functional chemicals with important properties in the field of health care, cosmetics, food and agriculture. Naringin composed of aglycon naringenin and the disaccharide neohespiridose, is the principle bitter flavanone glycoside and the primary bitter component in citrus fruits. Enzymatic hydrolysis is a possibility to overcome the bitterness. This study aimed to isolate Aspergillus sp.

    production-purification-and-application-of-naringinase-from-aspergillus-sp-sec-nigri
  • The microorganisms in biofilms live in a self-produced matrix of hydrated extracellular polymeric substances (EPS) that mainly comprises of polysaccharides, proteins, nucleic acids and lipids and provide mechanical stability to the biofilms by mediating their adhesion to surfaces and forming a cohesive, three-dimensional polymer network that interconnects and transiently immobilizes biofilm cellsThe mainaim of this study was to analyse the potential use of fungal enzymes like polysaccharidase

    studies-on-the-effect-of-fungal-enzymes-on-bacterial-biofilm-inhibition
  • In contrast to other fields in biology, mathematical thinking and methodology have become entrenched in neuroscience since its very beginning, as witnessed by the classical work of Hodgkin and Huxley. One of the biggest open challenges mathematicians and engineers face is to understand the complex computation that takes place in our brain. Among the most interesting parts of the brain, is the ‘little brain’, otherwise known as the cerebellum.

    computational-neuroscience-and-cognitive-modeling

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