COURSE NAME: Cell & Molecular Biology – Lab
COURSE CODE: BIO488
PROGRAM: MSc Bioinformatics
Isolation of plasmid DNA and genomic DNA; Protein extraction and purification by affinity chromatography and analysis by SDS-PAGE. Polymerase chain reaction (PCR). Microscopic observation of different types of cells: bacteria, animal cell, plant cell, fungi. Cell viability test by Trypan Blue. Image analysis (SDS-PAGE, cell morphology, tissue section) by ImageJ. Basics of animal cell culture.