DETAILS:

COURSE NAME: Cell & Molecular Biology – Lab
COURSE CODE: BIO488
CREDIT: 2
SEMESTER: 2
PROGRAM: MSc Bioinformatics

SYLLABUS

Isolation of plasmid DNA and genomic DNA; Protein extraction and purification by affinity chromatography and analysis by SDS-PAGE. Polymerase chain reaction (PCR). Microscopic observation of different types of cells: bacteria, animal cell, plant cell, fungi. Cell viability test by Trypan Blue. Image analysis (SDS-PAGE, cell morphology, tissue section) by ImageJ. Basics of animal cell culture.

References:

  1. Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).
  2. Amrita University Virtual Lab.
  3. Freshney, R. I. (2005). Culture of specific cell types. John Wiley & Sons, Inc.