DETAILS:

COURSE NAME: Microbiology Lab
PROGRAM: M.Sc. Biotechnology
SEMESTER: One
CREDITS: Two
INSTRUCTOR: Suja Subhash

Sterilization techniques-Dry heat, moist heat, filters sterilization and UV irradiation- Media preparation- Simple, selective and differential media- Pure culture technique-Serial dilution, pour plate, streak plate, spread plate-Staining techniques-Simple staining, Gram staining, acid fast staining, Negative staining, Spore staining- Biochemical reactions of microbes-IMViC, Carbohydrate fermentation, oxidase, catalase- Enumeration of microbes– serial dilution agar plate technique-Microscopic examination of living bacterial preparation- Studying the growth pattern of a microorganism.

TEXT BOOK:

Microbiology-A laboratory Manual-James G.Cappucino,Natalie Sherman.