DETAILS: 

COURSE NAME: Recombinant DNA Technology
PROGRAM: M.Sc. Biotechnology and  M.Sc. Microbiology 
SEMESTER: Two
CREDITS: Three
INSTRUCTOR: Dr. Nandita Mishra, Dr. Sanjay Pal

Syllabus

Enzymology of Genetic manipulation, Genome Editing, Cloning Vectors  and Method of Transformations, Gene Isolation Approaches, Gene expression in heterologous system, Techniques used in genetic engineering, Plant, animal transgenics for biotechnological application and gene therapy, Mutagenesis, Knock-in, Knock-out, conditional knock-outs, Regulation of gene expression, cDNA arrays, gene silencing by RNAi, dominant negative approach, in vivo and in vitro protein protein interactions, bacterial and yeast one, two and three hybrids, phage display,  GST pull down, co-immunoprecipitation, Far Western blot, FRET, Biacore; DNA, RNA – protein interactions, Applications of genetic engineering                                        

Text Books

  • Brown, T. (2010). Gene cloning and DNA analysis: an introduction. John Wiley & Sons.
  • Primrose, S. B., & Twyman, R. (2009). Principles of gene manipulation and genomics. Wiley. com.
  • Howe, C. J. (2007). Gene cloning and manipulation. Cambridge University Press.