Recombinant DNA Technology Lab Second Semester Syllabus for MSc Biotechnology and MSc Microbiology
Transformation methods, genomic DNA isolation, Plasmid DNA isolation, restriction digestion of Plasmid and genomic DNA, elution of DNA by low melting gel agarose, Ligation, insert analysis, isolation of RNA, northern blotting, PCR, RT-PCR, Recombinant protein expression, purification and refolding.
- Sambrook, J., Russell, D. W., & Russell, D. W. (2001). Molecular cloning: a laboratory manual (3-volume set).
- Amrita University Virtual Lab.