DETAILS: 

COURSE NAME:Analytical Biochemistry
PROGRAM: B.Sc. Biotechnology and B.Sc. Microbiology
CREDITS: Three
 

SYLLABUS

  • Methods of protein extraction, Protein quantitation: Biuret, Lowry, BCA and Bradford methods, Protein precipitation: Salting-in, Salting-out, Effect of organic solvents and polymers. Protein separation: Dialysis, Ultrafiltration, Centrifugation.
  • Chromatography: Partition coefficient, Retention, Resolution, Capacity factor, theoretical plate, van Deemter curve, Gel flitration chromatography, Ion exchange chromatography, Affinity chromatography, Hydrophobic interaction chromatography, Hydroxyapatite chromatography, Paper chromatography, Thin layer chromatography, Reversed-phase chromatography, Fundamentals of high performance chromatography.
  • Electrophoresis: Native PAGE, SDS-PAGE, Isoelectric focussing, 2D-PAGE, Capillary electrophoresis.
  • Spectroscopy: Fundamentals of UV Spectroscopy, Spectrophotometer, Fundamentals of fluorescence spectroscopy, Spectrofluorometer.
  • Mass Spectrometry: Electron Impact, Chemical Ionization and Fast atom Bombardment mass spectrometers.

 

REFERENCES

  1. Biochemistry: Voet and Voet
  2. Protein Purification Techniques: Simon Roe
  3. Protein Purification: Robert K. Scopes
  4. Physical Biochemistry: David Sheehan
  5. Practical Biochemistry: Keith Wilson and John Walker
  6. Mass Spectrometry for Biotechnology: Gary Siuzdak