DETAILS:

COURSE NAME: Enzyme Technology Lab
COURSE CODE: BIO283
PROGRAM: B.Sc. Biotechnology
SEMESTER: Four
CREDITS: Two
 

Syllabus

  1. Standard Maltose Curve
  2. Isolation of Alpha/Beta Amylase
  3. Determination of enzyme activity
  4. Construction of Protein standard curve by Folin’s Lowry method and Determination of specific activity of enzyme.
  5. Effect of substrate concentration on Enzyme kinetics and determination of Km and Vmax
  6. Effect of temperature on Enzyme kinetics
  7. Effect of time on Enzyme kinetics
  8. Effect of pH on Enzyme kinetics