DETAILS

COURSE NAME: General Microbiology Lab
COURSE CODE: MIC281
PROGRAM: B.Sc. BiotechnologyB.Sc. Microbiology
SEMESTER: Three
CREDITS: Two
 

LESSON PLAN

Prescribed Syllabus

Lab Precautions, Sterilization, Micrometry, Capsular Staining , Motility determination-Soft agar deeps and Hanging drop method, Biochemical tests: IMViC test,Urease test, Catalase test, Oxidase test, carbohydrate fermentation separately and Triple sugar iron test, Anaerobic culture techniques-Wrights tube method, Ribotyping (demo)
 

Sl. No. TOPICS TO BE COVERED NO. OF SESSIONS ALLOTED
1. Lab Precautions and Sterilization 3h
2. Pure Culture Techniques-Spread Plate and Pour Plate 3h
3. Micrometry 3h
4. Motility determination-Soft agar deeps and Hanging drop method 3h
5. Staining techniques- Gram staining 3h
6. Capsular Staining 3h
7. Biochemical tests: Indole and methyl red test 3h
8. Voges-Proskaur and Citrate test 3h
9. Catalase  and Oxidase test 3h
10. Urease test 3h
11. Triple sugar Iron Agar test + separate sugar fermentation tests 3h
12. Anaerobic culture techniques-Wrights tube method 3h
13. Ribotyping 3h

REFFERENCE AND TEXT BOOKS

  1. Microbiology Lab Manual by James G. Cappuccino and Natalia Sherman.
  2. Benson’s Microbiological Applications by Alfred E. Brown
  3. Amrita Virtual Lab

 

CONTINUOUS ASSESSMENT SCHEME (35 Marks)

  • Rough Record - 10 marks
  • Fair Record - 10 marks
  • Assignment - 5
  • Viva - 10 marks
  • End Semester Exam - 65
  • Attendance - 5 marks