DETAILS: 

COURSE NAME: Genetic Engineering
PROGRAM: B.Sc. Biotechnology
SEMESTER: Five
CREDITS: Four
 

SYLLABUS:

MOLECULAR TOOLS FOR GENE CLONING
Nucleases: Exonucleases and Endonucleases, Restriction Enzymes (Type I, Type II, Type III, Type IV & Type V), RNases
Methylases: CpG Methylase , Dam Methylase, Dcm Methylase
Polymerases: DNA Pol I, Klenow Fragments, Reverse Transcriptase, Taq & Pfu Polymerases
Ligases: T4 DNA Ligase, E.coli DNA Ligase, T4 RNA Ligase
Topoisomerases: Type I(A, B) & Type II(A,B)
End Modifying Enzymes: Terminal Transferase, T4 Polynucleotide Kinase, Alkaline Phosphatases 

VECTORS AND GENE CLONING
Introduction to cloning vectors -- Desirable properties of vectors – Prokaryotic & Eukaryotic Expression Systems (Constitutive & Inducible)
Plasmid Vectors -- Phage Vectors -- Cosmids -- Phagemids -- BACs -- Yeast Vectors -- YACs -- Lentiviral Vectors -- Adenoviral Vectors -- Plant Vectors
Insect Vectors (Optional Reading)

ADVANCED TECHNIQUES IN MOLECULAR BIOLOGY
Polymerase Chain Reaction -- Quantitative Real Time PCR -- Gel Electrophoresis: AGE & PAGE -- Blotting Techniques: Southern, Western & Northern
Methods of gene transfer in Plants and Animals: Chemical, Physical & Viral mediated DNA transfer
Construction of Genomic & cDNA Libraries -- DNA Sequencing -- Protein Engineering: Site Directed Mutagenesis -- Reporter Gene Assays -- DNA Protein Interactions: EMSA, DNA Footprinting -- Protein Protein Interactions: Y2H, Y3H, B1H, B2H 

RECENT TRENDS IN MOLECULAR BIOLOGY RESEARCH
Targeted Genome Editing: ZFNs, TALENs, CRISPRs -- Gene Targeting: Knock-ins & Knock-outs -- DNA Finger Printing 



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