DETAILS: 

COURSE NAME: Genetic Engineering Lab
COURSE CODE: BIO386
PROGRAM: B.Sc. Biotechnology and B.Sc. Microbiology
SEMESTER: Five
CREDITS: Two
 

SYLLABUS

  1. Isolation of Plasmid DNA by Alkaline lysis method
  2. Quantitation of DNA
  3. Detection of Plasmid DNA by Agarose gel electrophoresis
  4. Restriction Digestion Analysis
  5. Competent cell preparation
  6. Transformation and Efficiency of competent cells
  7. SDS PAGE
  8. Polymerase Chain Reaction
  9. Isolation of Genomic DNA from Plants
  10.  Calibration of pipettes