DETAILS:

COURSE NAME: Introductory Microbiology lab
COURSE CODE: MIC180
PROGRAM: B.Sc BiotechnologyB.Sc Microbiology
SEMESTER: One
CREDITS: Two
 

SYLLABUS:

Prescribed Syllabus

Sterilization techniques and principles, Media preparation-Liquid and Solid media-Agar deeps Slants, Agar plates, Cultural Characteristics, Pure Culture techniques-streaking, Motility determination-Soft agar deeps and Hanging drop method, Staining techniques: Simple staining, Gram staining, Endospore staining, 

Sl. No. Topics to be covered No. of sessions alloted
1. Regulations in the lab 1.5h
2. Methods of Sterilization 1.5h
3. Media preparation-Broth,deep, slant and plate 3h 
4. Pure Culture techniques- introduction and Streaking (Continuous and quadrant) 3h
5. Cultural Characteristics in broth and solid media 3h
6. Microscopy- introduction 3h
7. Motility determination-Soft agar deeps and Hanging drop method 3h
8. Staining techniques- Simple staining 3h
9. Negative Staining 3h
10. Gram staining 3h
11. Endospore Staining 3h


REFFERENCE AND TEXT BOOKS

  1. Microbiology Lab Manual by James G. Cappuccino and Natalia Sherman.
  2. Benson’s Microbiological Applications by Alfred E. Brown
  3. Amrita Virtual Lab

 

CONTINUOUS ASSESSMENT SCHEME (35 Marks)

  • Rough Record - 10 marks
  • Fair Record - 10 marks
  • Assignment - 5
  • Viva - 10 marks
  • End Semester Exam - 65