Introductory Microbiology First Semester Syllabus for BSc Biotechnology and BSc Microbiology
Basic concepts– Spontaneous generation, Germ theory of diseases, Cell theory. Contributions of Antonie van leuwenhoek, Joseph Lister, Robert Koch, Louis Pasteur, Edward Jenner, John Tyndall, Sergei N. Winogradsky, Selman A waksman, Alexander Flemming, Paul Erlich, Fannie Hesse, Elie Metchnikoff, Kary Mullis. Development of pure culture methods.Cell ultra structure: Peptidoglycan structure and synthesis.Cytoplasmic matrix and components: Inclusion bodies.
Sterilisation and disinfection- Definitions, Principles. Methods of sterilization- Physical methods (Heat, Filteration), Radiation and Chemical methods. Control of sterilization and Testing of sterility. Microscopy – Principles, Light microscope, Phase Contrast, Dark field, Bright field, Fluorescent, Interference microscope (Stereo microscope), Confocal, Inverted microscope, and Electron microscope (TEM and SEM). Measurement of Microorganisms- Micrometry. Staining- Simple, Gram staining, Negative staining, Capsule staining, Spore staining, Flagellar staining, Nuclear staining and Acid fast staining.
Microbiological media, composition and types: selective and differential media Growth curve and growth kinetics. Influence of environemental factors for microbial growth. Nutritional groups of bacteria: overview Estimation of Microbes- Direct Microscopic count, Turbidometric assay, TVC- Indirect Method- CO2 liberation- Protein estimation- Maintenance and Preservation of cultures
Taxonomy– Principle and its types (Classical approach– Numerical, Chemical, Serological and Genetic). Bacterial taxonomy– Bergey’s manual of Systematic Bacteriology (Eubacteria and Archaebacterium).
- Prescott, L.M J.P. Harley and C.A. Klein 1995. Microbiology 2nd edition Wm, C. Brown publishers.
- Michael J. Pelczar, Jr. E.C.S. Chan, Moel : Microbiology Mc Graw Hill Book R. Krieg, 1986 Company
- Stainer R.Y. Ingraham J.L. Wheolis H.H and Painter P.R. 1986 The Microbial world, 5th edition. Eagle Works Cliffs N.J. Prentica Hall.
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