DETAILS: 

COURSE NAME: Recombinant DNA Technology
PROGRAM: B.Sc. Microbiology
SEMESTER: Five
CREDITS: Two

SYLLABUS:

PART 1: The Basic Principles of Gene Cloning and DNA Analysis   Introduction, History, The advent and importance of gene cloning and the polymerase chain reaction, Vectors for Gene Cloning, Purification of DNA from Living Cells, Manipulation of Purified DNA, Introduction of DNA into Living Cells,

PART 2: Vectors for Cloning

Cloning Vectors for E. coli, λ and other high capacity vectors, Cloning Vectors for Eukaryotes, Genomics & cDNA Libraries

PART 3: Applications and Techniques of Gene Cloning  

Polymerase Chain Reaction & qPCR, Electrophoresis & Blotting Techniques, Site- Directed Mutagenesis, DNA Sequencing, Reporter Gene Assays, DNA-Protein Interaction Assays, Protein-Protein Interaction Assays, DNA Fingerprinting.

 

TEXT BOOKS

  1. Gene Cloning and DNA Analysis: An Introduction, 6th Edition, T. A. Brown, Wiley-Blackwel
  2. Principles of Gene Manipulation & Genomics – 7th Edition – Sandy B.Primrose, Richard Twyman– Blackwell