Instruments and Facilities available at Amrita School of Biotechnology
T100™ THERMAL CYCLER
It is used for Nucleic acid amplification ie, to amplify a gene of interest. PCR (Polymerase chain reaction) decreases the time needed for generating a pure sample of gene of interest. Gene isolation by PCR can only amplify genes with predetermined sequences. PCR can also used for the gene cloning and analysis, gene expression analysis, mutational analysis and for cycle sequencing.
7900HT FAST REAL-TIME PCR SYSTEM
7900HT system supports many real-time PCR applications such as
Viral Load Analysis
Reproducibility, specificity and speed are the three major requirements for PCR in any application. The Master cycler pro is unparalleled in its ability to fulfill these requirements.
These thermal cyclers feature extremely fast heating and cooling rates (up to 8°C/s with silver blocks), intuitive graphic programming, and a display to indicate cycler number in a network. It has a Triple Circuit heating technology with Fast, Standard, or Safe temperature control modes. Satellite System use allows us to control up to five units with one control panel or to expand to thirty units with a computer network using Cycle-Manager pro software.
THE STEPONEPLUS™ QPCR
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. Utilizing robust LED based 4-color optical recording, the PCR System is designed to deliver precise, quantitative qPCR results for a variety of research applications.
BIOLOGIC DUO FLOW CHROMATOGRAPHY SYSTEM
Fast protein liquid chromatography (FPLC) is a form of medium-pressure chromatography that uses a pump to control the speed at which the mobile phase passes through the stationary phase.
|High resolution purification of proteins, peptides and other biomolecules|
|Automated injection, buffer selection, column switching, and large volume fraction collection are easily performed using various valve configurations.|
|The system supports several sample loading methods and multiples samples can be loaded sequentially using Econo Gradient Pump with AVR9-8 as inlet valve.|
|The system supports the use of multiple columns and thus in column switching. Strong and weak anion and cation exchange columns, size exclusion chromatography columns, hydrophobic interaction chromatography columns are supported by the system.|
|Reverse flow chromatography is possible using the AVR7-3 inject valves, for certain applications such as affinity chromatography.|
The BD FACS Calibur™ platform allows users to perform cell analysis complemented by software solutions to streamline analysis for a wide range of applications including cell cycle, enumeration of lymphocyte subsets, residual white blood cells etc. and various other research purposes.
In biotechnology, flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second.
Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. A common variation is to physically sort particles based on their properties, so as to purify populations of interest.
IX71 INVERTED MICROSCOPE
The IX71 inverted research microscope is designed to accommodate a wide range of advanced research techniques. Some features include:
|Superior S/N ratio for imaging excellence.|
|High N.A. objectives for fluorescence imaging.|
|High transmittances over a wider wavelength range.|
BioPhotometer plus, offers a compact UV/Vis photometer for use in molecular biology, biochemistry and cell biology.
|Measurement of DNA, RNA and protein concentration (UV and colorimetric)|
|Incorporation rate of fluorescent molecules (550 nm/650 nm), e. g., for microarray experiments|
|Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase)|
|Optical density of cells (OD 600)|
GEL DOCTM XR+ SYSTEM
The Gel Doc XR+ System enables quick and easy visualization, documentation, and analysis of nucleic acid and protein gels, blots, and microarrays. The system supports fluorescence and colorimetric detection methods.
This can also used for Monoclonal and polyclonal antibody binding affinities, Gel and blot imaging, Colony counting, Immunoassay, Multiplex Protein Detection, Post-Translational Modification Characterization, 2D Electrophoresis, Protein Quantitation etc.
MULTIMODE MICROPLATE READER
Modular and reliable microplate multimode reader is very sensitive and robustness especially in luminescence and BRET measurements the reader supports all important reading technologies including;
|Luminescence||BRET and BRET²||Fluorescence (top and bottom)|
|FRET||Fluorescence Polarisation (FP)||UV/VIS Absorbance|
|AlphaScreen® and AlphaLISA®||Time-Resolved Fluorescence||HTRF®|
LABCONCOFREEZONE 2.5 FREEZE DRYER
The Lyophilizer used for fast cooling to –84°C. A bright LCD displays operating parameters, set up parameters and alarm messages. Vacuum can be displayed in mBar, Pa or Torr, and temperature in °F or °C. LED "waves" display relative system vacuum and collector temperature.
Wet samples can be frozen by placing them in a vacuum. The more energetic molecules escape, and the temperature of the sample falls by evaporative cooling, eventually it freezes. About 15% of the water in the wet material is lost. It consist of a vacuum chamber into which wet sample material could be placed, together with a means of removing water vapor so as to freeze the sample by evaporative cooling and freezing and then maintain the water-vapor pressure below the triple-point pressure. The temperature of the sample would then continue to fall below the freezing point and sublimation would slow down until the rate of heat gain in the sample.
GENE PULSERXCELLTM ELECTROPORATION SYSTEMS
Electroporator is used for transfecting every cell type. The system includes a main unit, a ShockPodTMcuvette chamber and choice of accessory modules; the capacitance extender (CE module) and the pulse controller (PC module).
Electroporation is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA. If bacteria and plasmids are mixed together, the plasmids can be transferred into the bacteria after electroporation. It highly efficient for the introduction of foreign genes into tissue culture cells, speciallymammalian cells. For example, it is used in the process of producing knockout mice, as well as in tumor treatment, gene therapy, and cell-based therapy.
Several hundred volts across a distance of several millimeters are typically used in this process.
The stereo or stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly different viewing angles to the left and right eyes. This arrangement produces a three-dimensionalvisualization of the sample being examined.Stereomicroscopy overlaps macro-photography for recording and examining solid samples with complex surface topography, where a three-dimensional view is needed for analyzing the detail.
The stereo microscope is often used to study the surfaces of solid specimens or to carry out close work like dissection.
Measure the concentration and purity of DNA, RNA or protein samples using the NanoDrop 2000 spectrophotometer.
Wide spectral range (190-840nm) for measuring a variety of samples types:
|Peptides (205nm)||DNA and RNA (260nm)|
|Purified protein (280nm)||Toxicology assays and industrial dyes (490nm)|
|Gold nanoparticles (520nm)||Optical Density measurements (600 nm)|
|Colorimetric protein assays (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)|
INVERTED PHASECONTRAST/FLUORESCENT MICROSCOPE
It reveals many cellular structures that are not visible with a simpler bright field microscope. The phase contrast microscope made it possible for biologists to study living cells and how they proliferate through cell division. With the help of fluorescent source it is possible to identify cells and cellular components with a high degree of specificity. For example, certain antibodies and disease conditions or impurities in inorganic material can be studied with the fluorescence microscopy. The technique is used to study specimens, which can be made to fluoresce.
MINI BEAD BEATER
The BeadBeater will disrupt over 90% of the cells in about 2-5 minutes of operation. The homogenization procedure involves cell 'cracking' action rather than high shear. It is used to isolate proteins, membranes or organelles. When isolating nucleic acids in aggressive extraction media containing phenol/chloroform, guanidinium salts, and/or detergents, temperature control is usually not necessary.