DETAILS:

COURSE NAME: Biochemistry Lab
COURSE CODE: BIO482
PROGRAM: M.Sc. Biotechnology and M.Sc. Microbiology
SEMESTER: One
CREDITS: Two
INSTRUCTOR: Dr. Jayashree G.Dr. Sobha V. Nair, Dr. Athira O.

SYLLABUS:

Preparation of Laboratory Solutions and Buffers [problems and Calculations],  Separation of plant pigments by column chromatography ,Thin layer chromatography , Preparation of standard curve  for the Enzyme Assay, Effect of substrate concentration on Enzyme kinetics and determination of Km and Vmax,  Estimation of protein and specific activity of the enzyme , Structural studies of phycobiliproteins from spirulina using  spectroscopic techniques , Protein purification [Ammonium sulphate precipitation, Affinity Chromatography and SDS PAGE],  Lab activity

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