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Lipopeptides in the triggering of erythrocyte cell membrane scrambling

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Cellular Physiology and Biochemistry

Source : Cellular Physiology and Biochemistry, Karger Publishers, Volume 22, Number 5-6, p.381–386 (2008)

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Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2008

Abstract : Sepsis is paralleled by anemia, an effect partially resulting from eryptosis, the suicidal death of erythrocytes. Eryptosis is characterized by cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. Pathogen-induced eryptosis may partially result from interaction of bacterial cell wall components such as lipoproteins with the erythrocyte cell membrane. The present study explored, whether the synthetic lipopeptide Pam3CSK4 mimicking the acylated amino terminus of bacterial lipoproteins triggers eryptosis. According to annexin-V-binding in FACS analysis, Pam3CSK4 (1 μg/ml) stimulated phosphatidylserine exposure, an effect significantly blunted in the nominal absence of Ca2+. According to Fluo3 fluorescence, Pam3CSK4 increased cytosolic Ca2+ activity and moderately stimulated erythrocytic ceramide formation, both major triggers of eryptosis. In conclusion, bacterial lipoproteins participate in the stimulation of erythrocyte cell membrane scrambling by bacterial cell wall components. Thus, lipoprotein-dependent suicidal erythrocyte death may contribute to the pleotropic effects of sepsis.

Cite this Research Publication : K. Wang, Mahmud, H., Föller, M., Dr. Raja Biswas, Lang, K. S., Bohn, E., Goetz, F., and Lang, F., “Lipopeptides in the triggering of erythrocyte cell membrane scrambling”, Cellular Physiology and Biochemistry, vol. 22, pp. 381–386, 2008.

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