Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Revista Iberoamericana de Micologia
Source : Rev Iberoam Micol, Volume 34, Issue 2, p.109-111 (2017)
Url : https://www.ncbi.nlm.nih.gov/pubmed/28392225
Keywords : agar, Candida, Candidiasis, Chromogenic Compounds, Culture Media, Drug Resistance, Multiple, Fungal, Humans, Mycological Typing Techniques, Species Specificity
Campus : Kochi
School : School of Medicine
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Microbiology, Nanosciences and Molecular Medicine
Year : 2017
Abstract : Background: Candida auris is unique due to its multidrug resistance and misidentification as Candida haemulonii by commercial systems. Its correct identification is important to avoid inappropriate treatments. Aims: To develop a cheap method for differentiating C. auris from isolates identified as C. haemulonii by VITEK2. Methods: Fifteen C. auris isolates, six isolates each of C. haemulonii and Candida duobushaemulonii, and one isolate of Candida haemulonii var. vulnera were tested using CHROMagar Candida medium supplemented with Pal's agar for better differentiation. Results: On CHROMagar Candida medium supplemented with Pal's agar all C. auris strains showed confluent growth of white to cream colored smooth colonies at 37. °C and 42. °C after 24 and 48. h incubation and did not produce pseudohyphae. The isolates of the C. haemulonii complex, on the contrary, showed poor growth of smooth, light-pink colonies at 24. h while at 48. h the growth was semiconfluent with the production of pseudohyphae. C. haemulonii complex failed to grow at 42. °C. Conclusions: We report a rapid and cheap method using CHROMagar Candida medium supplemented with Pal's agar for differentiating C. auris from isolates identified as C. haemulonii by VITEK2. © 2017 Asociación Española de Micología.
Cite this Research Publication : Anil Kumar, Sachu, A., Mohan, K., Vinod, V., Dinesh, K., and Karim, S., “Simple low cost differentiation of Candida auris from Candida haemulonii complex using CHROMagar Candida medium supplemented with Pal's medium.”, Rev Iberoam Micol, vol. 34, no. 2, pp. 109-111, 2017.