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Course Detail

Course Name Molecular Biology Lab
Course Code 24MMD581
Program M. Sc. Molecular Medicine(MLM)
Semester 1
Credits 2
Campus Faridabad, Kochi

Syllabus

Unit 1

Isolation of chromosomal DNA from Escherichia coli;Agarose gel electrophoresis, Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR), Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from Escherichia coli; cDNA synthesis, Reverse Transcriptase PCR, DNA sequencing, Real time PCR.

Objectives and Outcomes

Pre-requisites: Basic understanding of DNA chemistry and DNA biology

Total number of classes: 30

Course Outcome

CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.
CO2 Can isolate DNA, RNA, plasmids.
CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.
CO4 Can follow general safety routines for laboratory work in molecular biology.
CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.

*Program Outcomes (PO)(As given by NBA and ABET)

PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

2

2

1

3

1

–

1

3

–

–

1

CO 2

3

2

3

3

3

1

–

1

3

–

–

1

CO 3

3

2

3

3

3

1

–

1

3

–

–

1

CO 4

2

3

3

2

2

1

–

–

–

–

–

1

CO 5

3

3

1

2

3

2

–

–

–

–

–

1

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine 

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine 

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

C O

CO 1

2

–

1

3

1

2

–

–

–

1

CO 2

–

–

–

3

1

1

–

–

–

1

CO 3

–

–

–

3

1

1

–

–

–

1

CO 4

–

–

–

3

–

–

–

–

–

–

CO 5

–

–

–

3

–

–

–

–

–

–

Evaluation Pattern: 30+70 = 100

Internal Assessment – 30% 

Records 

Evaluation 

30% 

 

30%

End Semester Examination- 50% 

Practical 

Exam 

60% 

Assessment

Viva 

10%

 

70%

Text Books / References

Text book

  1. Joseph Sambrook, David William Russell, Molecular Cloning: A Laboratory Manual, Volume 1-2-3 CSHL Press, 2001.
  2. Terence A. Brown, Essential Molecular Biology: A Practical Approach, Volume 1 Oxford University Press, 2000.
  3. Udo Reischl; Molecular Diagnostics of infectious diseases; Humana Press. 
  4. Adrian J Harwood, Methods in Molecular Biology; Protocols for gene analysis.
  5. Frederick M Ausubel, Roger Brent, Robert D Moore, J G Seidman, John A smith, Kevin Struhl; Current protocols in Molecular Biology, John Wiley and Sons, Inc (Volume 1-4).

Reference

Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.

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