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Course Detail

Course Name Advanced Instrumentation Techniques
Course Code BP 811 ET
Program B. Pharm. (Regular/ Lateral)
Semester Eight
Year Taught 2014 , 2015 , 2016 , 2017 , 2018


Unit I

Nuclear Magnetic Resonance spectroscopy: Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications

Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ioniza- tion, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

(10 Hours)

Unit II

Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnaly- sis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, Xray Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.

(10 Hours)

Unit III

Calibration and validation-as per ICH and USFDA guidelines Calibration of following Instruments Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC

(10 Hours)

Unit IV

Radio immune assay: Importance, various components, Principle, different methods, Limitation and Applications of Radio immunoassay

Extraction techniques: General principle and procedure involved in the solid phase extraction and liquid liquid extraction

(08 Hours)

Unit V

Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.

(07 Hours)


‘Advanced Instrumentation Techniques’ is a course offered in the eighth semester of B. Pharm program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.

Scope & Objectives

Course Duration: 45 Hours


This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart advanced knowledge on the principles and instrumentation of spectroscopic and chromatographic hyphenated techniques. This also emphasiz- es on theoretical and practical knowledge on modern analytical instruments that are used for drug testing.


Upon completion of the course the student shall be able to

  1. Understand the advanced instruments used and its applications in drug analysis
  2. Understand the chromatographic separation and analysis of drugs.
  3. Understand the calibration of various analytical instruments
  4. Know analysis of drugs using various analytical instruments.


Recommended Books (Latest Editions)

  1. Instrumental Methods of Chemical Analysis by B.K Sharma
  2. Organic spectroscopy by Y.R Sharma
  3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
  4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
  5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
  6. Organic Chemistry by I. L. Finar
  7. Organic spectroscopy by William Kemp
  8. Quantitative Analysis of Drugs by D. C. Garrett
  9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
  10. Spectrophotometric identification of Organic Compounds by Silverstein

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