| Course Name | Clinical Biochemistry and Proteomics |
| Course Code | 22MMD512 |
| Program | M. Sc. Molecular Medicine(MLM) |
| Credits | 3 |
| Campuses | Faridabad, Kochi |
15 lectures
Carbohydrate and fatty acid metabolism, Glycolysis; TCA cycle, Oxidative
Phosphorylation – Energetics and its regulation; Cori cycle; Glycogen Metabolism; Pentose phosphate pathway; Gluconeogenesis pathway and significance; Biosynthesis of fatty acids; Oxidation of fatty acids – Beta oxidation, alpha oxidation.
8 lectures
Structures & function of enzymes, mechanism of action of enzymes and its regulation; Kinetics of enzyme catalyzed reactions, Michaelis-Menten equation, Importance of Vmax, Km; Enzyme inhibition and activation.
6 lectures
Concept and scope of clinical biochemistry; Control of the blood glucose and associated clinical diseases; Reference range.
6 lectures
Proteome and proteomics research, how it is different from genomics; different types of proteomics, significance of sample preparation in proteomics, significance of choosing different methods for proteome analysis, gel-free and gel-based proteome analysis, labelled and label-free quantitative proteomics.
10 lectures
Principles of mass spectrometry; protein identification using mass spectrometry, protein fragmentation; peptide enrichment and separation; ionization and its importance; Time of Flight, MS/MS analysis, types of mass analyzers, peptide fragmentation and peptide sequencing; identification of proteins using search engines/programs; accuracy of identified proteins with respect to protein identity, significance of mass spectrometry in clinics, clinical proteomics and examples of clinical proteomics
Pre-requisites: Basic understanding of biology, chemistry and biotechnology
Total number of classes: 45
Preamble
The course intends to provide a basic understanding of the biochemical reactions; role of macromolecules and enzymes that govern the biochemical transformations and biochemical mechanisms responsible for common biochemical disorders; mass-spectrometry based proteomics for protein identification and quantitation, application of proteomics in the clinics for aiding in diagnosis, prognosis and treatment of diseases.
CO1: Understand overall concept of cellular metabolism, energy storage and release, enzymes and their regulation CO2: Understand glucose homeostasis (pathways and hormonal regulation); glycogen metabolism, gluconeogenic pathway, fat metabolism. CO3: Understand the basic concepts and principles of clinical biochemistry and molecular mechanism of some common biochemical disorders CO4
CO1: Understand overall concept of cellular metabolism, energy storage and release, enzymes and their regulation
CO2: Understand glucose homeostasis (pathways and hormonal regulation); glycogen metabolism, gluconeogenic pathway, fat metabolism.
CO3: Understand the basic concepts and principles of clinical biochemistry and molecular mechanism of some common biochemical disorders
CO4: Understand the basic concepts of proteome, mass spectrometry and protein identification using database search engines.
CO5: Discuss how proteomics can contribute to a clinical setting.
Programme Outcomes (PO) (As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 | |
| CO1 | 3 | 1 | 1 | 1 | – | – | – | – | – | – | – | 1 |
| CO2 | 3 | 1 | 1 | – | – | – | – | – | – | – | – | 1 |
| CO3 | 3 | 2 | 1 | 2 | – | 2 | – | – | – | – | – | 1 |
| CO4 | 3 | 1 | 2 | 2 | 3 | – | – | – | – | – | – | 1 |
| CO5 | 3 | 1 | 2 | 3 | 3 | 2 | – | – | – | – | – | 1 |
Program Specific Outcomes. (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular dysregulation in diseases
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 | |
| CO1 | 3 | 2 | 1 | – | – | – | – | – | – | – |
| CO2 | 3 | 2 | 1 | – | – | – | – | – | – | – |
| CO3 | 1 | 3 | 3 | 2 | 1 | – | – | – | – | – |
| CO4 | – | 1 | 1 | 3 | – | – | – | – | – | 3 |
| CO5 | – | 2 | 1 | 3 | – | – | – | – | – | 3 |
Evaluation Pattern: 50+50 = 100
| Internal Assessment – 50% | ||
| Periodical 1 | Exam | 20% |
| Periodical 2 | Exam | 20% |
| Continuous Assessment | Assignment/ Test/ Quiz | 10% |
| 50% | ||
| End Semester Examination- 50% | ||
| Theory Exam | 50% | |
| 50% | ||
| Total | 100% | |
Textbooks:
Reference:
Lehniger Principles of biochemistry 7th Edition (2017), Publisher: WH Freeman, Authors Michael Cox, David L. Nelson.
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