Syllabus
Unit I
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications – Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry
Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications
(10 Hours)
Unit II
IR spectroscopy
Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations
Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Ther- mocouple, Thermister, Pyroelectric detector and applications
Flame Photometry -Principle, interferences, instrumentation and applications
Atomic absorption spectroscopy – Principle, interferences, instrumentation and applications
Nepheloturbidometry – Principle, instrumentation and applications
(10 Hours)
Unit III
Introduction to chromatography
Adsorption and partition column chromatography – Methodology, advantages, disadvantages and applications.
Thin layer chromatography – Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
Paper chromatography -Introduction, methodology, development techniques, advantages, disadvantages and applications
Electrophoresis – Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capil- lary electrophoresis, applications
(10 Hours)
Unit IV
Gas chromatography – Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications
High performance liquid chromatography (HPLC) – Introduction, theory, instrumentation, advantages and applications.
(08 Hours)
Unit V
Ion exchange chromatography – Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications
Gel chromatography – Introduction, theory, instrumentation and applications
Affinity chromatography – Introduction, theory, instrumentation and applications
(07 Hours)
Summary
‘Instrumental Methods of Analysis – Theory’ is a course offered in the seventh semester of B. Pharm program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.
Scope & Objectives
Course Duration: 45 Hours
Scope:
This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart a fundamental knowledge on the principles and in- strumentation of spectroscopic and chromatographic technique. This also emphasizes on theoretical and practical knowledge on modern analytical instruments that are used for drug testing.
Objectives:
Upon completion of the course the student shall be able to:
- Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis
- Understand the chromatographic separation and analysis of drugs.
- Perform quantitative & qualitative analysis of drugs using various analytical instruments.
