| Course Name | Introductory Microbiology lab |
| Course Code | MIC 180 |
| Program | BSc. in Biotechnology, BSc. in Microbiology |
| Semester | One |
| Credits | Two |
| Year Taught | 2019 |
Sterilization techniques and principles, Media preparation-Liquid and Solid media-Agar deeps Slants, Agar plates, Cultural Characteristics, Pure Culture techniques-streaking, Motility determination-Soft agar deeps and Hanging drop method, Staining techniques: Simple staining, Gram staining, Endospore staining,
| Sl. No. | Topics to be covered | No. of sessions alloted |
| 1. | Regulations in the lab | 1.5h |
| 2. | Methods of Sterilization | 1.5h |
| 3. | Media preparation-Broth,deep, slant and plate | 3h |
| 4. | Pure Culture techniques- introduction and Streaking (Continuous and quadrant) | 3h |
| 5. | Cultural Characteristics in broth and solid media | 3h |
| 6. | Microscopy- introduction | 3h |
| 7. | Motility determination-Soft agar deeps and Hanging drop method | 3h |
| 8. | Staining techniques- Simple staining | 3h |
| 9. | Negative Staining | 3h |
| 10. | Gram staining | 3h |
| 11. | Endospore Staining | 3h |
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