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Course Detail

Course Name Laboratory Techniques in Translational Research I
Course Code 24TM507
Program M.Sc. in Translational Medicine
Semester I
Credits 2
Campus Faridabad


Unit 1:
Introduction to Laboratory Techniques in Translational Research, Overview of translational medical research, Importance of laboratory techniques in translational research, Laboratory safety practices and regulations
Unit 2:
Basic Laboratory Skills and Equipment Operation, Laboratory pipetting techniques, Measurement and calibration of laboratory equipment, Use of laboratory instruments (centrifuges, spectrophotometers, etc.)
Unit 3:
Molecular Biology Techniques, DNA/RNA extraction and purification methods, Polymerase chain reaction (PCR) and real-time PCR, Gene cloning and recombinant DNA technology
Unit 4:
Cell Culture Techniques, Cell line maintenance and culture, Primary cell isolation and culture, Cell proliferation, viability, and assays

Objectives and Outcomes


Laboratory Techniques in Translational Medical Research is a graduate-level course designed to provide students with hands-on experience in the laboratory techniques commonly used in translational medical research. The course will cover essential laboratory skills, experimental design, data analysis, and interpretation relevant to translational research in medicine. Through laboratory sessions, demonstrations, lectures, and practical exercises, students will gain proficiency in various molecular, cellular, and biochemical techniques essential for conducting translational research.

Course outcome

CO1: To acquire proficiency in a variety of laboratory techniques commonly used in translational medical research.
CO2: To understand the principles underlying each laboratory technique, including experimental design, data analysis, and interpretation.
CO3: To learn about the application of laboratory techniques in studying disease mechanisms, biomarker discovery, and therapeutic development.
CO4: To develop critical thinking skills in experimental design, troubleshooting, and result interpretation.
CO5: To gain hands-on experience in laboratory safety practices, equipment operation, and data documentation.
CO6: To apply laboratory techniques to address specific research questions relevant to translational medicine.

Program outcome (PO)

PO1: Utilize scientific principles and methodologies to design innovative solutions for data analysis, experimentation, and product development for challenges in translational research.
PO2: Recognize the importance of environmental sustainability in translational research and strive to minimize adverse environmental impacts.
PO3: Engage in ethical conduct, leadership, active listening, constructive feedback, and interpersonal communication to facilitate productive collaborations and knowledge exchange.
PO4: Acquire fundamental and advanced knowledge and skills in project management, financial planning, and entrepreneurship relevant to translational research ventures and initiatives.
3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

CO 1 3 1 3
CO 2 3 1 3
CO 3 3 1 3
CO 4 3 1 3
CO 5 3 3 3 3
CO 6 3 1 3

Program Specific Outcome (PSO)

PSO1: Addresses the complexity of interdisciplinary sciences in biological and medical contexts.

PSO2: Deals with regulatory affairs in medicine, covering topics such as ethical considerations and regulatory frameworks.

PSO3: Covers compounds as drugs and their efficacy, involving pharmacology and drug development.

PSO4: Explores the intersection of bioinformatics and artificial intelligence in biology and medicine.

PSO5: Deals with technology in personalizing medicine, involving precision medicine approaches.

PSO6: Focuses on communicating and disseminating science and medicine to the public, involving science communication and public outreach efforts.

CO 1 3 1 1 3
CO 2 3 3 2 2
CO 3 3 3 3 3 3
CO 4 3 1 1 2
CO 5 3 3 1 1 1
CO 6 3 2 3 1 1


Alberts, B., 2017. Molecular biology of the cell. (5th Edition) Garland science.
Lodish, H.F., 2008. Molecular cell biology. (7th Edition) Macmillan.

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