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Course Detail

Course Name Microbiology Lab
Program M. Sc. in Biotechnology
Semester One
Credits Two
Year Taught 2019


Sterilization techniques-Dry heat, moist heat, filters sterilization and UV irradiation- Media preparation- Simple, selective and differential media– Pure culture technique-Serial dilution, pour plate, streak plate, spread plate-Staining techniques-Simple staining, Gram staining, acid fast staining, Negative staining, Spore staining– Biochemical reactions of microbes-IMViCCarbohydrate fermentationoxidasecatalase– Enumeration of microbes– serial dilution agar plate technique-Microscopic examination of living bacterial preparation– Studying the growth pattern of a microorganism

Text Books

  1. Microbiology-A laboratory Manual-James G.Cappucino,Natalie Sherman.


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