Course

‘Pharmaceutical Microbiology – Practical’ is a course offered in the second year of Pharm. D. program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.

Course Duration: 3 Hrs./Week

List of Experiments:

1. Study of apparatus used in experimental microbiology*.
2. Sterilisation of glass ware’s. Preparation of media and sterilisation.*
3. Staining techniques – Simple staining ; Gram’s staining ; Negative staining**
4. Study of motility characters*.
5. Enumeration of micro-organisms (Total and Viable)*
6. Study of the methods of isolation of pure culture.*
7. Bio chemical testing for the identification of microorganisms.*
8. Cultural sensitivity testing for some micro-organisms.*
9. Sterility testing for powders and liquids.*
10. Determination of minimum inhibitory concentration.*
11. Microbiological assay of antibiotics by cup plate method.*
12. Microbiological assay of vitamins by Turbidometric method**
13. Determination of RWC.**
14. Diagnostic tests for some common diseases, Widal, malarial parasite.**
    * Indicate minor experiment & ** indicate major experiment

Assignments:

Format of the assignment:

Scheme of Practical Examination

Note : Total sessional marks is 30 (20 for practical sessional plus 10 marks for regularity, promptness, viva-voce and record maintenance).

1. Visit to some pathological laboratories & study the activities and equipment/instruments used and reporting the same.
2. Visit to milk dairies (Pasteurization) and microbial laboratories (other sterilization methods) & study the activities and equipment/instruments used and reporting the same.
3. Library assignments
   1. Report of recent microbial techniques developed in diagnosing some common diseases.
   2. Latest advancement developed in identifying, cultivating & handling of microorganisms.
   1. Minimum & Maximum number of pages.
   2. It shall be computer draft copy.
   3. Reference(s) shall be included at the end.
   4. Name and signature of the student.
   5. Assignment can be a combined presentation at the end of the academic year.
   6. Time allocated for presentation may be 8+2 Min.